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KMID : 0370220060500060367
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Yakhak Hoeji
2006 Volume.50 No. 6 p.367 ~ p.371
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Inhibition of Nitric Oxide Synthesis by Ergosterol Derivative from Phellinus pini in LPS-Activated RAW 264.7 Cells
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Jang Hyun-Jin
Yang Ki-Sook
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Abstract
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Phellinus pini (Hymenocaetaceae) has been used for the immunomodulating activity, hypolipidemic effect, gastric cancer, non-insulin dependant diabetes, diarrhea, and menstrual irregularity. From the screening of each fraction of the inhibitory activity of NO production in lipopolysaccaride (LPS) activated RAW 264.7 cells, methanol extract of phellinus pini and hexane soluble fraction exhibited inhibition of NO production compared with LPS control without toxicity. The hexane soluble raction showed dose dependent inhibition of NO production. According to activity guided fractionation, the active hexane fr. was repeatedly chromatographed over silica gel, ergosta-4,6,8(14),22-tetraen-3-one was isolated. The compoundinhibited NOS activation (IC50:29.7uM) and NO production of activated macrophage at 30 uM.
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KEYWORD
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Phellinus pini, ergosta-4, 6, 8(14), 22-tetraen-3-one, nitric oxide, RAW 264.7cells
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